3/30/2023 0 Comments Wingren study center![]() ![]() However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. Lectins are widely used to analyze the cell surface expression of SA. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. 14, no 8, article id 1875 Article in journal (Refereed) Published Abstract John Wiley & Sons, 2022 KeywordsAR pathway and antibody-based therapy, FcγRIIIa receptor, PIP5K1α, prostate cancer metastasis, targeted therapy Thus, FcγRIIIa may serve as a potential new target for improved treatment of metastatic and castration-resistant PCa. Further, the elevated activation of FcγRIII and AR and PIP5K1α pathways may cooperatively promote PCa growth and invasion. Our study identified FcγRIIIa as an important factor in promoting PCa growth and invasion. FcγRIIIa physically interacted with PIP5K1α and AR via formation of protein-protein complexes, suggesting that FcγRIIIa is functionally associated with AR and PIP5K1α in PCa cells. ![]() Similarly, inhibition of PIP5K1α decreased FcγRIIIa expression in PCa cells. ![]() We further showed that elevated expression of AR enhanced FcγRIIIa expression, whereas inhibition of AR activity using enzalutamide led to a significant downregulation of FcγRIIIa protein expression. Conversely, targeted inhibition of FcγRIIIa via siRNA-mediated knockdown or using its inhibitory antibody suppressed growth of xenograft PC-3 and PC-3M prostate tumors and reduced distant metastasis in xenograft mouse models. We demonstrated that overexpression of FcγRIIIa increased the proliferative ability of PCa cell line C4-2 cells, which was accompanied by the upregulation of androgen receptor (AR) and phosphatidylinositol-4-phosphate 5-kinase alpha (PIP5Kα), which are the key players in controlling PCa progression. PCa patients with an elevated level of FcγRIIIa expression had poorer biochemical recurrence (BCR)-free survival compared with those with lower FcγRIIIa expression, suggesting that FcγRIIIa is of clinical importance in PCa. In this study, we found that FcγRIIIa expression was present in PCa cells and its level was significantly higher in metastatic lesions than in primary tumors from the PCa cohort (p=0.006). However, the role of FcγRIIIa in prostate cancer (PCa) progression remained unknown. Low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa) is a cell surface protein that belongs to a family of Fc receptors that facilitate the protective function of the immune system against pathogens. 2496-2517 Article in journal (Refereed) Submitted Abstract Place, publisher, year, edition, pagesīasel: MDPI, 2022 Keywordscell morphology CD44 circulating tumor cell colorectal cancer digital holographic cytometry EpCAM Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). In this study, we intended to mimic the environment for metastasizing cells. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. 9, no 12, article id 955 Article in journal (Refereed) Published Abstract Ĭolorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. ![]()
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